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il 15  (R&D Systems)


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    Structured Review

    R&D Systems il 15
    Il 15, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+conjugated+anti+il+15/Human+IL-15+PE-conjugated+Antibody/pm41372583-513-56-58
    Average 91 stars, based on 13 article reviews
    il 15 - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    Magnetic Beads:

    Article Title: Efficient production and enhanced tumor delivery of engineered extracellular vesicles
    Article Snippet: .. EV-loaded magnetic beads were then washed using a magnetic tube stand, and divided for staining with individual fluorophore-conjugated antibodies: FITC-lectin (provided in kit) and PE-conjugated anti-IL-15 (clone 34559; R&D Systems, Minneapolis, MN). ..

    Staining:

    Article Title: Efficient production and enhanced tumor delivery of engineered extracellular vesicles
    Article Snippet: .. EV-loaded magnetic beads were then washed using a magnetic tube stand, and divided for staining with individual fluorophore-conjugated antibodies: FITC-lectin (provided in kit) and PE-conjugated anti-IL-15 (clone 34559; R&D Systems, Minneapolis, MN). ..

    Article Title: T cells surface-loaded with immunostimulatory fusion molecules and uses thereof
    Article Snippet: .. After 1 hr incubation at 37° C. cells were washed two times in full media, followed by staining with PE-conjugated anti-IL-15 and Alexa-Fluor-647-conjugated anti-IL-21 antibodies (R&D Systems cat. no. IC2471P and BioLegend cat. no. 513006). .. Cells were analyzed on a FACSCelesta flow cytometer using DiVa software, and data were analyzed using Cytobank.

    Flow Cytometry:

    Article Title: NK cells provide helper signal for CD8+ T cells by inducing the expression of membrane-bound IL-15 on DCs.
    Article Snippet: NK cell recognition of cells that do not express or express low amounts of MHC class I molecules results not only in direct killing of target cells but also in the generation of specific T cell responses consequent to the induction of dendritic cell (DC) activation.. While IL-12 production by NK cellactivated DCs is generally thought to play a critical role, a similar DC-mediated NK cell help has been reported also in IL-12-knockout mice.. Here, we show that human NK cells can induce on DC surface membrane, via IFN-g secretion, the expression of high levels of IL-15.

    Immunofluorescence:

    Article Title: NK cells provide helper signal for CD8+ T cells by inducing the expression of membrane-bound IL-15 on DCs.
    Article Snippet: NK cell recognition of cells that do not express or express low amounts of MHC class I molecules results not only in direct killing of target cells but also in the generation of specific T cell responses consequent to the induction of dendritic cell (DC) activation.. While IL-12 production by NK cellactivated DCs is generally thought to play a critical role, a similar DC-mediated NK cell help has been reported also in IL-12-knockout mice.. Here, we show that human NK cells can induce on DC surface membrane, via IFN-g secretion, the expression of high levels of IL-15.

    Incubation:

    Article Title: T cells surface-loaded with immunostimulatory fusion molecules and uses thereof
    Article Snippet: .. After 1 hr incubation at 37° C. cells were washed two times in full media, followed by staining with PE-conjugated anti-IL-15 and Alexa-Fluor-647-conjugated anti-IL-21 antibodies (R&D Systems cat. no. IC2471P and BioLegend cat. no. 513006). .. Cells were analyzed on a FACSCelesta flow cytometer using DiVa software, and data were analyzed using Cytobank.



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